Greater Department of Biological Sciences,
Osogbo, Osun State
Nigeria
Research Article
In vitro Antimicrobial Activities of Vigna subterranea
Author(s): Ajiboye AA and Oyejobi GKAjiboye AA and Oyejobi GK
The antibacterial assay of the seed of Vigna subterranea (Bambara Groundnut) on certain clinical isolates: Proteus mirabilis, Escherichia coli, Klebsiella aerogenes, Klebsiella ozaenae, Staphylococcus aureus and Pseudomonas aeruginosa was determined using the agar diffusion technique to investigate its potential use as antibacterial agent. The antimicrobial activity of the test organisms to the seed extract was screened by using the agar-well diffusion method. An inoculum suspension was swabbed uniformly to solidified 20 ml Mueller-Hinton Agar (MHA) and the inoculum was allowed to dry for 5 minutes. Holes of 5 mm in diameter were made in the seeded agar using sterile cork-borer. Aliquot of 50 μl from each seed extract was added into each well on the seeded medium and allowed to stand on the bench for 1 hour for proper diffusion .. Read More»
DOI:
10.4172/2472-1212.1000132
Journal of Antimicrobial Agents received 444 citations as per Google Scholar report