Journal of Cytology & Histology

Paola Boarelli

Publications

• Research Article
  Simultaneous Study of Cholesterol and GM1 Ganglioside by Specific Probes: Lipid Distribution during Maturation, Capacitation and the Acrosome Reaction
  Author(s): Paola Boarelli, Monclus M, Lancellotti TS, Cabrillana M, Simón L, Funes A and Fornés MPaola Boarelli, Monclus M, Lancellotti TS, Cabrillana M, Simón L, Funes A and Fornés M
  
  Lipid distribution has been extensively studied in the plasma membrane of the male gamete due to its relevant role in sperm physiology. However, there are controversial reports about their distribution. Because of different reports about this topic, the aim of this work was define cholesterol and GM1 ganglioside location by simultaneous detection during sperm maturation, capacitation and the acrosome reaction in CF1 mouse sperm. We observed that throughout the epididymis, cholesterol detected by filipin III or complex and GM1 ganglioside by cholera toxin, colocalized in the acrosomal domain of the head and midpiece of the flagellum. During sperm capacitation, a decrease of cholesterol can be observed like diffuse pattern on the whole head, while GM1 ganglioside was restricted to the apical acrosome. Capacitated sperm incubated with acrosome reaction inducers showed a diffuse location .. Read More»
  DOI: 10.4172/2157-7099.1000412

  Abstract PDF