Cancer Science & Therapy

Patricia Granja Coelho

Publications

• Research Article
  Methodological Standardization for the Extraction of Free DNA in Plasma of Peripheral Blood
  Author(s): Ary Serpa Neto, Marcelo Langer Wroclavski, Jorge Luiz Freire Pinto, Sarah Rodrigues Marsicano, Pamela Oliveira Delgado, Patricia Granja Coelho, Ricardo Moreno, Viviane Aparecida Vilas Boas, Ligia Ajaime Azzalis, Virginia Berlanga Campos Junqueira, Auro Del Giglio and Fernando Luiz Affonso FonsecaAry Serpa Neto, Marcelo Langer Wroclavski, Jorge Luiz Freire Pinto, Sarah Rodrigues Marsicano, Pamela Oliveira Delgado, Patricia Granja Coelho, Ricardo Moreno, Viviane Aparecida Vilas Boas, Ligia Ajaime Azzalis, Virginia Berlanga Campos Junqueira, Auro Del Giglio and Fernando Luiz Affonso Fonseca
  
  Background: Biological materials such as cells, DNA, RNA, and proteins can be recovered from blood, urine, feces, pancreatic juice and sputum of patients. Here, we described a method for free plasma DNA extraction used in our laboratory, compared it to one of the most reproduced in the literature, and also verified the effects of short time storage of plasma on DNA quantification. Methods: We assessed DNA concentrations in four samples of peripheral blood one hour, one day and three days after plasma separation (part A). EDTA blood (10 mL) was collected from each individual (10) and the specimen was centrifuged at 1,300 g for 10 min. The supernatant was transferred into polypropylene tubes, with particular attention not to disturb the buffy coat layer and the plasma was microcentrifuged at 2,400 g. DNA extracted.. Read More»
  DOI: 10.4172/1948-5956.S5-005

  Abstract PDF