Biosensors & Bioelectronics

Paul A Millner

Publications

• Research Article
  Midland Blotting: A Rapid, Semi-Quantitative Method for Biosensor Surface Characterization
  Author(s): Jo V Rushworth, Asif Ahmed and Paul A MillnerJo V Rushworth, Asif Ahmed and Paul A Millner
  
  Biosensor performance and readout are critically dependent upon sensor surface characteristics. It is vital that key steps in sensor construction, such as base layer polymer/Self-Assembled Monolayer (SAM) deposition and bioreceptor tethering, are controlled tightly in order to achieve high sensitivity, specificity and reproducibility. Here, we present a rapid, semi-quantitative method by which key biosensor surface features can be characterised using chemiluminescence. This technique, which we have termed midland blotting, permits the detection of biosensor surface components through the attachment of a HorseRadish Peroxidase (HRP)-conjugated reagent to the target of interest. Upon addition of luminol-based substrate, HRP generates a reagent which emits light when it decays. The light signal is proportional to the bound HRP on the sensor surface. We show here that midland blotting all.. Read More»
  DOI: 10.4172/2155-6210.1000146

  Abstract PDF