Journal of Bioprocessing & Biotechniques

Picollet-D’hahan N

Publications

• Research Article
  Culture Conditions can Counterbalance the Thermosensitive Properties of Poly (N-Isopropylacrylamide) during Cell Harvesting
  Author(s): Abeille F, Vinjimore Kesavan S, Dolega ME, Gerbaud S, Allier C, Pouteau P, Icard B, Agache V, Gidrol X and Picollet-D’hahan NAbeille F, Vinjimore Kesavan S, Dolega ME, Gerbaud S, Allier C, Pouteau P, Icard B, Agache V, Gidrol X and Picollet-D’hahan N
  
  Poly (N-isopropylacrylamide) (PNIPAM) has been widely referenced as an alternative solution for proteolyticenzyme- based cell harvesting. However, the reversibility (i.e., consecutive attachment-detachment cycles) of PNIPAM for cell harvesting has not been studied. In this work, we synthesized PNIPAM-grafted glass surfaces to study the efficiency of fibroblastic cell detachment after iterative use. For robust quantification of thermally triggered cell detachment, an innovative wide-field lens free video microscope was introduced. The cell detachment efficiency was shown to decrease significantly after each cell culture experiment due to the loss of polymer brush thermosensitivity. The influence of the temperature variation rate on cell detachment from the re-used PNIPAM-grafted surfaces was further characterized. The cell detachment behavior on these subst.. Read More»
  DOI: 10.4172/2155-9821.1000217

  Abstract PDF