Journal of Bioanalysis & Biomedicine

Pooja R Patel

Publications

• Research Article
  Norepinephrine Reduces Reactive Oxygen Species (ROS) and DNA Damage in Ovarian Surface Epithelial Cells
  Author(s): Pooja R Patel, Muralidhar L Hegde, Jacob Theruvathu, Sankar A Mitra, Istvan Boldogh and Lawrence SowersPooja R Patel, Muralidhar L Hegde, Jacob Theruvathu, Sankar A Mitra, Istvan Boldogh and Lawrence Sowers
  
  Objective: To determine the role of norepinephrine (NE) on DNA damage and reactive oxygen species (ROS) generation in ovarian surface epithelial cells. Method: Non-tumorigenic, immortalized ovarian surface epithelial cells were treated with NE, bleomycin, and bleomycin followed by NE. The comet assay was performed on each treatment group to determine the amount of single and double-strand breaks induced by treatments. ROS levels for each treatment group were measured using the H2DCF-DA fluorescence assay. Finally, RNA transcripts were measured for each treatment group with regards to the expression of DNA repair and oxidative stress genes. Results: The mean tail moment of untreated cells was significantly greater than that of cells t.. Read More»
  DOI: 10.4172/1948-593X.1000127

  Abstract PDF