Journal of Bioprocessing & Biotechniques

Qinzhu Zeng

Publications

• Research Article
  Characterization and Immobilization of Purified Alliinase Produced from Shallots
  Author(s): Qinzhu Zeng, Aihui Veng, Linghui Kong and Shan HeQinzhu Zeng, Aihui Veng, Linghui Kong and Shan He
  
  In this study, Alliinase extracted and purified from shallots was comprehensively examined. Its molecular weight was determined as 50 kDa, and its optimal working conditions were identified as pH 8 and 40ºC. Its activity was significantly and positively affected by the metals K+, Na+, Ga2+, Mg2+ and Fe2+. Purified Alliinase was immobilized by alginate and the optimized conditions for this process were: a sodium alginate concentration of 2.5%, a CaCl2 concentration of 4.0%, a Glutaraldehyde concentration of 0.75%, and a ratio of sodium alginate solution and Alliinase solution (by volumn) of 4:3. The ability for free Alliinase and immobilized Alliinase to release flavours from shallots was compared. It was found that immobilization did not compromise Alliinase’s flavor releasing ability. They both released 36 flavouring chemicals from the flavouring precursor, with a similar .. Read More»
  DOI: 10.4172/2155-9821.1000308

  Abstract PDF