Journal of Computer Science & Systems Biology

Raghunath Satpathy

Publications

• Research Article
  Homology Modelling of Lycopene Cleavage Oxygenase: The Key Enzyme of Bixin Production
  Author(s): Raghunath Satpathy, Rajesh Ku Guru, Rashmiranjan Behera and Aparajita PriyadarshiniRaghunath Satpathy, Rajesh Ku Guru, Rashmiranjan Behera and Aparajita Priyadarshini
  
  Bixin is a natural dye and a high commercial important compound, produced from Bixin synthetic pathway in case of Bixa orellana plant. The particular enzyme Lycopene cleavage Oxygenase catalyzes the fi rst step of reaction pathways from Trans-lycopene to Bixin synthesis. The 3D structure of the enzyme was predicted by MODELLER program and the missing side chains were verifi ed by SCRWL4 tool. Model validation was done by using the output of PROCHECK and DOPE score. The Ramachandran plot for the model was observed as 87.3 percentages of residues is in favourable regions that indicate the model is reliable. Molecular dynamics simulation of the model protein was performed in water 5 lakh times for 1000 Pico second and at 300K by GROMACS software in a high performance computing environment. The energy value and RMSD (Root men square deviation) fl uctuation of .. Read More»
  DOI: 10.4172/jcsb.1000057

  Abstract PDF