Saudi Arabia
Research Article
Estimation of Swertiamarin in Enicostemma Littorale and Marketed Formulations Using HPLC-UV Method
Author(s): Prawez Alam, Mohammad Ali, Rahul Singh and Faiyaz ShakeelPrawez Alam, Mohammad Ali, Rahul Singh and Faiyaz Shakeel
A simple, economic, reproducible, robust and precis e HPLC method for estimation of swertiamarin in both 60 % methanolic extract of Enicostemma littorale and mar- keted formulations was developed and validated in p resent investigation. The mobile phase composed of methano l and water (90:10 % v/v), gave a sharp and well-defi ned peak of swertiamarin at the retention time of 10.15 ± 1.52 min. The limit of detection (LOD) and limit of quan tifica- tion (LOQ) were 17.25 and 56.92 μ g/ml respectively. The proposed method with high degree of precision and a ccu- racy was employed for the estimation of swertiamari n in methanolic extract and in marketed formulation... Read More»
DOI:
10.4172/1948-593X.1000005
Journal of Bioanalysis & Biomedicine received 3099 citations as per Google Scholar report