Journal of Bioanalysis & Biomedicine

Rajesh Dhiman

Publications

• Research Article
  Determination of S-amlodipine in Human Plasma Using Tizanidine as Internal Standard by Lc/Ms/Ms Method
  Author(s): Rajesh Dhiman, Vijaya Durga and Konsam Hamlet MeeteiRajesh Dhiman, Vijaya Durga and Konsam Hamlet Meetei
  
  High performance liquid chromatographic tandom mass spectrometric method for the estimation of S-amlodipine in human plasma has been developed and validated using Tizanidine as internal standard. Sample process was accomplished by Liquid-Liquid Extraction technique. The processed sample was chromatographed and analysed on Phenomenex, Lux 3u Cellulose-2 (150 × 4.6) mm column using mobile phase [0.1% formic acid in water and Acetonitrile (50:50% v/v)]. S-amlodipine was chromatographed and analysed by MS detector. Amlodipine has two basic moiety (one primary and one secondary amino) so it shows pKa=9.45 with two ester linkages and one ether linkage, so it is highly lipid soluble (logP=2.22) whereas Tizanidine has two secondary amino and three tertiary amino groups, so it shows pKa=7.49. Primary amine –NH2 is aliphatic in nature in amlodipine and se.. Read More»
  DOI: 10.4172/1948-593X.1000130

  Abstract PDF