Brazil
Research Article
Influence of Breed and Individual Variation in the Quality of Frozen Canine Semen
Author(s): Rebeca Marques Mascarenhas and Rego de Paula TarcÃÂzio AntonioRebeca Marques Mascarenhas and Rego de Paula TarcÃzio Antonio
The present work aims to report the individual and racial difference found in the freezing of canine semen, as well as to analyze the correlation of the parameters of in vitro evaluation of semen before and after freezing. For this, 36 ejaculates were collected of 12 dogs of the Beagle, schnauzer, Doberman and Boxer breeds, by the digital manipulation method and frozen in Tris-Citrate medium containing 6% of glycerol. Semen was evaluated, before and after freezing, on sperm movement through vigor, motility and spermatic index, and on the integrity and viability of the spermatic membrane by hypoosmotic and supravital staining tests. Sperm longevity was estimated in the thawed semen through the thermoresistance test. Individual variation was observed on spermatic index in the thawed semen, but not in the fresh. The parameters of spermatic membrane integrity and viability presented indiv.. Read More»
DOI:
10.4172/2157-7579.1000498
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