Veterinary Science & Technology

Ricardo Seiti Yamatogi

Publications

• Research Article
  The Use of Real-Time PCR (qPCR) for the Diagnosis of Bovine Herpes virus 5 in Formalin-Fixed, Paraffin-Embedded Bovine Brain Samples Identified as Bovine Unspecific Encephalitis
  Author(s): Francisco Pedraza-Ordoñez, Ricardo Seiti Yamatogi, Joao Pessoa Araujo Jr., Noeme Sousa Rocha and Antonio Carlos AlessiFrancisco Pedraza-Ordoñez, Ricardo Seiti Yamatogi, Joao Pessoa Araujo Jr., Noeme Sousa Rocha and Antonio Carlos Alessi
  
  Background: Bovine herpes virus 5 (BoHV-5) is a neurovirulent alpha herpes virus that causes fatal meningoencephalitis and huge economic losses in the livestock and associated industries worldwide; its diagnosis in the laboratory is essential for disease control, immunologic surveillance and effective monitoring programs in endemic areas. Materials and Methods: In this study, brain samples from 21 Colombian cattle that died of neurological disease, were negative for rabies and were diagnosed with nonspecific encephalitis were processed by real-time PCR (qPCR) in the laboratory of virology at the Institute of Biosciences, Sao Paulo State University, Brazil, to detect BoHV-5 genetic material in tissue preserved in paraffin blocks. Tissues were considered positive for BoHV-5 if ampliï¬Âcation occurred at or before 30 PCR cycles.. Read More»
  DOI: 10.4172/2157-7579.1000145

  Abstract PDF