Journal of Bioengineering & Biomedical Science

Richard Thiele

Publications

• Research Article
  Extracorporeal Shockwaves (ESW) Promote Proliferation and Differentiation of Keratinocytes In vitro-Histology and Immunohistochemistry
  Author(s): Vlado Antonic, Bernd Hartmann, Sandra Münch, Claudia Belfekroun, Gerald Niedobitek, Beate Petschke, Mark Smith D, Richard Thiele, Wolfgang Schaden and Christian OttomannVlado Antonic, Bernd Hartmann, Sandra Münch, Claudia Belfekroun, Gerald Niedobitek, Beate Petschke, Mark Smith D, Richard Thiele, Wolfgang Schaden and Christian Ottomann
  
  The objective of presented study was to evaluate in vitro effects of extracorporeal shockwave therapy (ESWT) on the keratinocyte morphology, cytoskeleton and mitotic activity. To determine in vitro effects of ESWT on keratinocytes, we applied 100 pulses with an energy flux density 0.1 mJ/mm2 and a frequency of 1 Hz at a distance of 5 cm between therapy head and culture flask. The treatment parameters were determined in a pilot study. Haematoxylin and Eosin staining, as well as immunohistochemistry for Ki-67, CK5, CK14 and CK10 was performed on the cultured keratinocytes and results were compared between ESW treated group and Controls. No pathomorphological abnormalities or alterations in the cells monolayer could be observed. When assessing histological images of all HE-stained sections of ESW-treated and untreated keratinocytes (control group), no morphological differences could be s.. Read More»
  DOI: 10.4172/2155-9538.1000161

  Abstract PDF