Journal of Biometrics & Biostatistics

Robert H Podolsky

Publications

• Research Article
  Quantifying and Normalizing Methylation Levels in Illumina Arrays
  Author(s): Duchwan Ryu, Hongyan Xu, Varghese George, Shaoyong Su, Xiaoling Wang and Robert H PodolskyDuchwan Ryu, Hongyan Xu, Varghese George, Shaoyong Su, Xiaoling Wang and Robert H Podolsky
  
  The role of genome-wide patterns of methylation in human disease has drawn attention increasingly in recent  years, because the methylome has the potential for large effects in disease etiology. Most analyses of methylation have utilized the percent signal that is methylated, known as β-value, or the logistic transformation of β, named M-value, as the summary measures. However, in general, these summary measures do not follow a Normal distribution and lead to statistical tests sensitive to outlying samples. In this paper, we proposed the N-value, a type of weighted logistic transformation of β that accounts for signal variability among beads for analyses of differential methylation. Our analysis of 27K Illumina array data showed that the N-value follows a desirable shape of sample distribution, an.. Read More»
  DOI: 10.4172/2155-6180.1000164

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