Journal of Cytology & Histology

Roberto Giardino

Publications

• Research Article
  Matricellular Protein Expression and Cell Ultrastructure as Parameters to Test In Vitro Cytotoxicity of a Biomimetic Scaffold
  Author(s): Sandra Durante, Gabriella Teti, Viviana Salvatore, Stefano Focaroli, Monica Mattioli-Belmonte, Concetta Ferretti, Adriana Bigi, Roberto Giardino and Mirella FalconiSandra Durante, Gabriella Teti, Viviana Salvatore, Stefano Focaroli, Monica Mattioli-Belmonte, Concetta Ferretti, Adriana Bigi, Roberto Giardino and Mirella Falconi
  
  Following scaffold implantation, cell sufferance, in-vivo encapsulation, foreign body reaction and inflammatory response has been reported and the up- regulation of matricellular proteins is often connected with this condition. Cytotoxicity of biomaterials is generally tested according to ISO standard 10993-5 based mainly on viability tests. Additional assays, based on improved cytotoxicity knowledge, are suggested in order to better analyze the biocompatibility of implant materials. The purpose of the study was to evaluate the matricellular protein expression as biomarker for in vitro-testing the biocompatibility of implant materials. Tenascin-C, osteocalcin and osteopontin belong to the matricellular protein family and were chosen as cytotoxicity markers. Mesenchymal stem cells were seeded on collagen/hydroxyapatite scaffold and on carboxymethyl cellulose based hydrogel in order to .. Read More»
  DOI: 10.4172/2157-7099.1000262

  Abstract PDF