Journal of Cytology & Histology

Roselis Silveira Martins da Silva

Publications

• Research Article
  A Simple Method to Quantify Glycogen from Human Platelets
  Author(s): Débora Santos Rocha, Samir Khal de Souza, Tor Gunnar Hugo Onsten, Roselis Silveira Martins da Silva and Marcos Emílio FrizzoDébora Santos Rocha, Samir Khal de Souza, Tor Gunnar Hugo Onsten, Roselis Silveira Martins da Silva and Marcos Emílio Frizzo
  
  Objective: The aim of our study was to establish a protocol to quantify the glycogen from human platelets. Methods: The total glycogen was determined in the platelet concentrate (PC) obtained from healthy male volunteers. The assay comprised the following steps: (1) cell lysing and glycogen extraction, (2) precipitation of glycogen and its washing, (3) conversion of glycogen to free glucose, (4) neutralization, and (5) detection of free glucose. Results: In our analysis, the measured glycogen content in the PC was 0.263 ± 0.101 μg/106 platelets (mean ± SD, n=16). Discussion: Despite the potential importance of glycogen determination in the platelets, a biochemical method to quantify this polymer in these cells is not available at the moment. Our protocol proved to be a simple method to measure these reserves from a blood sample. We expect to contribute to future studi.. Read More»
  DOI: 10.4172/2157-7099.1000217

  Abstract PDF