Journal of Tissue Science and Engineering

Rudra Bhowmick

Publications

• Research Article
  Design of a Novel Method for the Spatial Distribution of Cells within a Porous Scaffold for Tissue Engineering Applications
  Author(s): Revathy Subramanian, Rudra Bhowmick and Heather Gappa-FahlenkampRevathy Subramanian, Rudra Bhowmick and Heather Gappa-Fahlenkamp
  
  Tissue engineering is rapidly progressing to provide complex, three-dimensional (3D) representations of human tissues that can be used for tissue replacement and/or to study tissue systems. Tissue engineering includes the addition of cells within 3D scaffolds, along with bioactive components, sometimes within a bioreactor. A major challenge in developing many tissue-engineered models is the ability to evenly distribute cells throughout a porous scaffold, in order to achieve good cell viability and growth. In this study, we created a 3D collagen-chitosan scaffold with specific properties to aid in seeding cells within the entire volume and investigated a dynamic method to seed cells within such scaffold. Based on the requirements for cell seeding, the scaffolds were less than 500 µm thick, had pore sizes greater than 50 µm and had a porosity of 50% or greater. Fibroblasts wer.. Read More»
  DOI: 10.4172/2157-7552.1000201

  Abstract PDF