Biosensors & Bioelectronics

Rupali Saxena

Publications

• Review Article
  Recent Techniques for the Detection of β-Thalassemia: A Review
  Author(s): Gourav Mishra, Rupali Saxena, Amit Mishra and Archana TiwariGourav Mishra, Rupali Saxena, Amit Mishra and Archana Tiwari
  
  β-thalassemias are very heterogeneous in nature, with respect to the molecular basis of the disease. This type of disorder is caused by extensive deletions occurring within the β-globin gene locus, present on chromosome number 11. Due to point mutations, stop codons in the β-globin mRNA is introduced, thus curbing its normal function. This paper reviews conventional and latest β-thalassemia detection techniques. Amongst the conventional techniques, the DNA-based piezoelectric biosensors were based on the observation of distinguishable frequency shifts, resulting from the hybridisation between an oligonucleotide probe which was immobilized on a gold electrode of a quartz crystal and the complementary strand; present in solution. Q-primer real time PCR (Q-PCR) system utilises the 5’>3’ exonuclease activity of a DNA polymerase, which cleaves the n.. Read More»
  DOI: 10.4172/2155-6210.1000123

  Abstract PDF