Journal of Bioprocessing & Biotechniques

Rwiti Basuthakur

Publications

• Research Article
  Optimization of Solid State Fermentation Conditions and Characterization of Thermostable Alpha Amylase from Bacillus subtilis (ATCC 6633)
  Author(s): Sujan Maity, Sanghamitra Mallik, Rwiti Basuthakur and Suvroma GuptaSujan Maity, Sanghamitra Mallik, Rwiti Basuthakur and Suvroma Gupta
  
  Alpha amylase production using microbial source and solid state fermentation has been conducted for past few years in search of thermostable enzyme. Owing to the prolific use of thermostable alpha amylase in various industries like paper, food, detergent, brewing and starch liquefaction process, the production of alpha amylase is still going on. In the present work, Bacillus subtilis (ATCC 6633) has been utilized for generation of alpha amylase followed by optimization of the fermentation media. Change in fermentation conditions like fermentation hour, temperature, inoculums size, nitrogen and sugar sources have pivotal role to enhance alpha amylase yield. The thermal, pH and detergent stability of partially purified alpha amylase have been tested and compared with purified porcine pancreatic amylase. The result is encouraging with approximate 80% retentio.. Read More»
  DOI: 10.4172/2155-9821.1000218

  Abstract PDF