Biosensors & Bioelectronics

Sadhna Singh

Publications

• Research Article
  Development of Surface Plasmon Resonance (SPR) Based Immuno-Sensing System for Detection of Fungal Teliospores of Karnal Bunt (Tilletia indica), a Quarantined Disease of Wheat
  Author(s): Sadhna Singh, Manoj Singh, Gohar Taj, Sanjay Gupta and Anil KumarSadhna Singh, Manoj Singh, Gohar Taj, Sanjay Gupta and Anil Kumar
  
  This paper first time describes Surface Plasmon Resonance (SPR) based immuno-sensing system for detection of fungal teliospores of Karnal Bunt (KB) disease of wheat incited by Tilletia indica. The results of present study were compared with the results of lab scale ELISA developed earlier in our lab. The approach involves the use of a rabbit polyclonal (anti-teliospore) antibody and a SPR sensor for label-free detection of teliospores from the model organism Tilletia indica. When the interaction between binder (anti-teliospore antibody) and teliosporic antigen(s) as analyte occurs at the sensor surface, a sensorgram was obtained in real time by plotting the signal against time. Under optimised assay conditions as few as 5 teliospores were detected by ELISA which justifies the greater affinity of raised anti-teliospore antibodies for its antigen. The sensitivity of each immunosensor co.. Read More»
  DOI: 10.4172/2155-6210.1000125

  Abstract PDF