Cancer Science & Therapy

Said M Abdou

Publications

• Research Article
  Genetic Variations of Selected Genes Using Target Deep Sequencing in Colorectal Cancer Patients
  Author(s): Eman E Farghal, Marwa H Saied, Fatma M Ghaith, Gamal I Moussa, Gehan El-Sharnobi, Samah M Soliman, Hesham Tawfik, Omnia Abdelfattah, Enas A Abdul-Baki, Fatma Gharib, Lamis Mohamed, Mohamed R El- Shanshory, Amira Y Abdelnaby, Mohamed L Salem, Mona Watany, Abdel Aziz A Zidan, Yahia S Abdou and Said M AbdouEman E Farghal, Marwa H Saied, Fatma M Ghaith, Gamal I Moussa, Gehan El-Sharnobi, Samah M Soliman, Hesham Tawfik, Omnia Abdelfattah, Enas A Abdul-Baki, Fatma Gharib, Lamis Mohamed, Mohamed R El- Shanshory, Amira Y Abdelnaby, Mohamed L Salem, Mona Watany, Abdel Aziz A Zidan, Yahia S Abdou and Said M Abdou
  
  Background: Colorectal carcinoma (CRC) is a burden problem in a developing country like Egypt since patients are usually admitted in late stage with bad prognosis and short overall survival. Because of genetic predisposition of CRC and introduction of advanced molecular techniques, efforts are directed to screen for potential pathogenic or disease-causing variants in CRC patients Methods: DNA was isolated from formalin fixed paraffin embedded tissue sections collected from 24 CRC confirmed diagnosed patients. TruSight CRC panel (Illumina) was used for detection of different variants in 15 genes. The generated reads were obtained from Illumina Miseq were clustered into single nucleotide polymorphism (SNPs) and small insertions/deletions (Indels). Further pathogenic variants with somatic and germline mutations were identified according to the .. Read More»
  DOI: 10.4172/1948-5956.1000492

  Abstract PDF