Molecular Biomarkers & Diagnosis

Saira Saleem

Publications

• Research Article
  Stem Cell Organoids in Primary Cultures of Human Non-Malignant and Malignant Colon
  Author(s): Sahrish Tariq, Muhammad Tahseen, Mariam Hassan, Muhammad Adnan Masood, Shahid Khattak, Aamir Ali Syed, Asad Hayat Ahmad, Mudassar Hussain, Muhammed Aasim Yusuf, Chris Sutton and Saira SaleemSahrish Tariq, Muhammad Tahseen, Mariam Hassan, Muhammad Adnan Masood, Shahid Khattak, Aamir Ali Syed, Asad Hayat Ahmad, Mudassar Hussain, Muhammed Aasim Yusuf, Chris Sutton and Saira Saleem
  
  Aims: A sub-population of cells named cancer stem cells (CSCs) that initiate and promote tumour growth have been demonstrated to exist in several malignancies including colon carcinoma. The objective of our pilot study was to isolate CD133+CD26+CD44+ CSCs from patient colon tumours, culture spheres or organoids and observe their proliferation in primary cultures. Parallel cultures of non-cancer controls from colon normal lining and nonadenomatous polyps were set up. Methods: Magnetic activated cell sorting was used to isolate CD133+CD26+CD44+ cell populations followed by primary cell culturing under stem cell culture conditions. Number, cells/organoid and daughter generations of organoids were calculated using phase contrast microscope. Trypan blue exclusion method was used to test the viability of the cells. Results: .. Read More»
  DOI: 10.4172/2155-9929.1000346

  Abstract PDF