Veterinary Science & Technology

Sampath Kumar B

Publications

• Research Article
  Production of Gentamycin and Ceftiofur Specific Polyclonal Antibodies by Conjugating them with Bovine Serum Albumin
  Author(s): Sampath Kumar B, Vasili Ashok, Kalyani P, Eswar Prasad P and Krishnaiah NSampath Kumar B, Vasili Ashok, Kalyani P, Eswar Prasad P and Krishnaiah N
  
  Polyclonal antibodies (pAbs) were produced against gentamycin (GEN) and ceftiofur (CEF) antibiotics in sprague dawley rats (aged 7-8 weeks) by conjugating them with bovine serum albumin (BSA). Total 3 groups (3 rats in each group): one control (without antibiotic) group and two test groups (GEN and CEF) of rats were maintained. Total of four blood samplings were done from each group as follows: First three samples was serially at 15 days’ time interval after 1st booster, 2nd booster, 3rd booster and the 4th sampling was one and half month after the third booster. The antibody titres in the antisera of each antibiotic in all the four immunization cycles were determined by an icELISA at various serum dilutions ranging from 1/100 to 1/6400. Analysis of antibiotic conjugates by SDS-PAGE and Coomassie blue staining revealed higher molecular weights when compared to normal BSA (68 KDa.. Read More»
  DOI: 10.4172/2157-7579.1000314

  Abstract PDF