Journal of Bioanalysis & Biomedicine

SatyaVani

Publications

• Research Article
  Study of Pharmacokinetics and Tissue Distribution of BITS-17 in Rat Plasma and Tissue Homogenate Using a Validated LC Method
  Author(s): Addepalli Venkata Ramani, Vadlamani Lakshmi Indira, SatyaVani, Sandeep Gill, Perumal Yogeeswari and Dharmarajan SriramAddepalli Venkata Ramani, Vadlamani Lakshmi Indira, SatyaVani, Sandeep Gill, Perumal Yogeeswari and Dharmarajan Sriram
  
  Accurate, simple, sensitive, reproducible and specific liquid chromatographic method has been developed for estimation of BITS-17 in rat plasma and other biological tissues using β-naphthol as an internal standard (IS). The applicability of the validated method was tested to estimate the concentrations of BITS-17 (a novel anti tubercular agent) in plasma and tissues that were harvested in a rat tissue distribution study. A liquid - liquid extraction technique was used to extract BITS-17 and internal standard from the plasma and various tissue homogenates. The main pharmacokinetic parameters obtained after intravenous administration were t½=14 ± 0.20 h, Kel=0.62 ± 0.10 h-1, mean plasma clearance=1.35 ± 0.16 mg/h and mean volume of distribution = 1.99 ± 0.49L. The systemic absorption was tested with two formulations was slow after oral admi.. Read More»
  DOI: 10.4172/1948-593X.1000067

  Abstract PDF