Journal of Tissue Science and Engineering

Seung-Hyun Hong

Publications

• Research Article
  Computer-Automated Static, Dynamic and Cellular Bone Histomorphometry
  Author(s): Seung-Hyun Hong, Xi Jiang, Li Chen, Pujan Josh, Dong-Guk Shin and David RoweSeung-Hyun Hong, Xi Jiang, Li Chen, Pujan Josh, Dong-Guk Shin and David Rowe
  
  Dynamic and cellular histomorphometry of trabeculae is the most biologically relevant way of assessing steady state bone health. Traditional measurement involves manual visual feature identification by a trained and qualified professional. Inherent with this methodology is the time and cost expenditure, as well as the subjectivity that naturally arises under human visual inspection. In this work, we propose a rapidly deployable, automated, and objective method for dynamic histomorphometry. We demonstrate that our method is highly effective in assessing cellular activities in distal femur and vertebra of mice which are injected with calcein and alizarin complexone 7 and 2 days prior to sacrifice. The mineralized bone tissues of mice are cryosectioned using a tape transfer protocol. A sequential workflow is implemented in which endogenous fluorescent signals (bone mineral, green and red.. Read More»
  DOI: 10.4172/2157-7552.S1-004

  Abstract PDF