Journal of Bioanalysis & Biomedicine

Sheetal Bandhu

Publications

• Research Article
  Influence of Cytosolic Malic Enzyme in Oleaginous Yeast Rhodotorula mucilaginosa IIPL32 for Lipid Biosynthesis
  Author(s): Sheetal Bandhu, Tripti Sharma, Deepti Agrawal, Debashish Ghosh, Dilip K Adhikari and Diptarka DasguptaSheetal Bandhu, Tripti Sharma, Deepti Agrawal, Debashish Ghosh, Dilip K Adhikari and Diptarka Dasgupta
  
  A cytosolic NADP+ dependent malic enzyme has been purified and characterized from an oleaginous yeast Rhodotorula mucilaginosa IIPL32 to investigate its role in lipid biosynthesis. The enzyme has selective and high affinity for NADP and L-malate. Sesamol, a nonoil component of sesame seed oil, has been used during nitrogen depleted condition to evaluate inhibition of malic enzyme and lipid production. Sesamol did not inhibit purified malic enzyme. Sesamol was converted into catechol like metabolite to impart enzyme inhibition and thus lipid production by decreased supply of NADPH+... Read More»
  DOI: 10.4172/1948-593X.1000161

  Abstract PDF