IR Iran
Research Article
Optimization of Submerged Culture Conditions for Mycelial Growth and Extracellular Polysaccharide Production by Coriolus versiolor
Author(s): Feifei Wang, Jianchun Zhang, Limin Hao, Shiru Jia, Jianming Ba and Shuang NiuFeifei Wang, Jianchun Zhang, Limin Hao, Shiru Jia, Jianming Ba and Shuang Niu
This paper is concerned with optimization of submerged culture conditions for mycelial growth and Exopolysaccharides (EPS) yield with Coriolus versiolor by both one-factor-at-a-time and orthogonal matrix methods. Glucose and yeast-Extracts were identified to be the most suitable carbon and nitrogen sources, respectively. The optimal initial pH, inoculum size and liquid volume for mycelial growth and EPS yield were 5.0, 8% and 150mL/500mL, respectively. Subsequently, the concentration of glucose, yeast-extract, KH2PO4, and MgSO4•7H2O were optimized using the orthogonal matrix method. The effects of media composition on the mycelial growth of Coriolus versiolor were in the order of glucose >KH2PO4 > yeast-extract > MgSO4•7H2O, and those on EPS .. Read More»
DOI:
10.4172/2155-9821.1000124
Journal of Bioprocessing & Biotechniques received 3351 citations as per Google Scholar report