Journal of AIDS & Clinical Research

Shoji Yamaoka

Publications

• Research Article
  Establishment of In-House Quantitative Real-Time RT-PCR Assay for HIV-1 Viral Load Measurement: Application to Evaluate Efficacy of ART in Ghanaian Patients in an Urban Setting
  Author(s): Jacob Samson Barnor, Norio Yamamoto, James Ashun Mensah Brandful, William Ampofo, Joseph Humphrey Kofi Bonney, Evelyn Bonney, John Kofi Odoom, Simeon Aidoo, Michael Alale, Nana Afia Ntim, Yaw Owusu Amoah, Sampson Badu Ofori, Jerry Ndzinu, Ishmael Dzigbordi Aziati, Nii-Akwei Addo, Alexander Nyarko, Eiji Ido, Koichi Ishikawa and Shoji YamaokaJacob Samson Barnor, Norio Yamamoto, James Ashun Mensah Brandful, William Ampofo, Joseph Humphrey Kofi Bonney, Evelyn Bonney, John Kofi Odoom, Simeon Aidoo, Michael Alale, Nana Afia Ntim, Yaw Owusu Amoah, Sampson Badu Ofori, Jerry Ndzinu, Ishmael Dzigbordi Aziati, Nii-Akwei Addo, Alexander Nyarko, Eiji Ido, Koichi Ishikawa and Shoji Yamaoka
  
  The aim of this study was to establish and apply a real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) for human immunodeficiency virus (HIV) RNA quantification in patients on antiretroviral treatment (ART) in Ghana, where recombinant strains including CRF02_AG are prevalent. The primers and TaqMan probe concentrations as well as reaction temperatures were optimized to establish an efficient in-house quantitative assay system for HIV RNA, a tool for HIV viral load measurement in patients. Then an already established HIV-specific PCR amplicon (HIV-1 NL4-3) was used as an external standard to estimate the linearity, amplification efficiency, analytical sensitivity and reproducibility of the in-house real time quantitative assay. Finally, the assay was applied to quantify the viral load in clinical samples of HIV patients on ART. The real time quantitative ass.. Read More»
  DOI: 10.4172/2155-6113.1000305

  Abstract PDF