Journal of Bioprocessing & Biotechniques

Shubham Banerjee

Publications

• Research Article
  Exploring Packaged Microvesicle Proteome Composition of Chinese Hamster Ovary Secretome
  Author(s): Niraj Kumar, Dixat Gopal Gupta, Srikant Kumar, Priyanka Maurya, Ashutosh Tiwari, Babu Mathew, Shubham Banerjee, Sagarika Haldar, Jonathan Pillai, Shinjini Bhatnagar and Susmita ChaudhuriNiraj Kumar, Dixat Gopal Gupta, Srikant Kumar, Priyanka Maurya, Ashutosh Tiwari, Babu Mathew, Shubham Banerjee, Sagarika Haldar, Jonathan Pillai, Shinjini Bhatnagar and Susmita Chaudhuri
  
  Background: Chinese Hamster Ovary cells (CHO) are the most preferred host cells to meet the increasing demand for high quality ‘human-like’ complex biologics production, but is faced with the challenge of achieving high yield at an affordable price. Secreted proteins critically impact cell growth and product quality and quantity and an integral part of secretome is the packaged microvesicles. In spite of numerous efforts to characterize spent-media proteome, none have identified specific contribution of microvesicles, necessitating further differential analysis of these defined fractions of spent-media proteome, specifically packaged microvesicles. Methods: We have investigated proteome of microvesicles isolated from lag, log, stationary and death phase of CHO batch culture using LC-MS/MS based-proteomic approach to identify proteins that .. Read More»
  DOI: 10.4172/2155-9821.1000274

  Abstract PDF