Journal of Cytology & Histology

Siham K Abunasef

Publications

• Research Article
  The Role of Combined Nicotine and Caffeine Administration on the Histological Structure of the Rat Urinary Bladder with a Special Reference to P53
  Author(s): Alhan I Allehaibi, Siham K Abunasef and Azza I ZakiAlhan I Allehaibi, Siham K Abunasef and Azza I Zaki
  
  Background: It is difficult to find microscopic studies that can demonstrate the effect of combined administration of nicotine and caffeine on the mucosa of the rat urinary bladder. Aim of work: So, the current study aimed at demonstration of histological and p53 immuno-histochemical expression of the mucosa of rat urinary bladder as a marker sharing in process of apoptosis following the combined administration of nicotine and caffeine. Materials and methods: The animals were divided randomly into 4 groups: control, Nicotine, caffeine and combined Nicotine and Caffeine. The urinary bladder was processed and stained with hematoxylin and eosin stain, histochemically by PAS and immunohistochemically by Anti p53 antibodies. Results: After nicotine there was apparent increase in the layers of epithelium with intraepithelial lymphocytes. The strong PAS positive reaction of the basal layer t.. Read More»
  DOI: 10.4172/2157-7099.1000455

  Abstract PDF