Journal of Bioprocessing & Biotechniques

Si-Liang Zhang

Publications

• Research Article
  Enhance Vitamin B12 Production by Online CO2 Concentration Control Optimization in 120 m3 Fermentation
  Author(s): Ze-Jian Wang, Hui-Yuan Wang, Ping Wang, Yi-ming Zhang, Ju Chu, Ying-Ping Zhuang and Si-Liang ZhangZe-Jian Wang, Hui-Yuan Wang, Ping Wang, Yi-ming Zhang, Ju Chu, Ying-Ping Zhuang and Si-Liang Zhang
  
  Great amounts of carbon dioxide generated by Pseudomonas denitrificans during high aerobic vitamin B12 fermentation, while the influence of CO2 concentration on vitamin B12 production remains unclear. In this paper, we present parallel experiments to investigate various levels of inlet CO2 fractions on the physiological metabolism of P. denitrificans in laboratory scale fermentation. The results demonstrated that the oxygen transfer rate, cell growth and glucose consumption were inhibited with CO2 fraction elevated from 0.03% to 8.86 ± 0.24%, while the most exciting results showed that the specific vitamin B12 production rate and the yield to glucose were greatly stimulated when dissolved CO2 increased to 8.86 ± 0.24%. Therefore, the optimal exhausted CO2 fraction contro.. Read More»
  DOI: 10.4172/2155-9821.1000159

  Abstract PDF