Journal of Bioanalysis & Biomedicine

Singh S. P

Publications

• Research Article
  Determination of Curcumin in Rat Plasma by Liquid–liquid Extraction using LC–MS/MS with Electrospray Ionization: Assay Development, Validation and Application to a Pharmacokinetic Study
  Author(s): Singh S. P, Wahajuddin and Jain G. KSingh S. P, Wahajuddin and Jain G. K
  
  A simple, speci fi c and rapid LC–MS/MS method has been developed and validated for the estimation of curcumin in rat plasma, using biochanin as internal standard (IS). The assay procedure involved liquid–liquid extraction of curcumin and IS from rat plasma. The recovery of curcumin and IS in rat plasma were 87.62 and 88.25%, respectively. The resolution of peaks was achieved with 0.01 M ammonium acetate (pH 5.5):acetonitrile (10:90, v/v) on a Supelco Discovery C18 column. The total chromatographic run time was 4 min. Speci fi city and matrix effect on ionization was determined and found that method was speci fi c and there was no signi fi cant matrix effect. The method was proved to be accurate and precise at linearity range of 10–2000 ng/mL with a correlation coef fi cient (r) of ≥ 0.995. The MS/MS ion transitions monitored were 367 .. Read More»
  DOI: 10.4172/1948-593X.1000027

  Abstract PDF