Thailand
Research Article
Deactivation Study of �Ž�±-Amanitin Toxicity in Poisonous Amanita spp. Mushrooms by the Common Substances In Vitro
Author(s): Paitoon Narongchai and Siripun NarongchaiPaitoon Narongchai and Siripun Narongchai
The purpose of this research was to find out the substance which deactivate α-Amanitin Toxicity
The materials and methods used in the study include analysis with high performance liquid chromatography (HPLC) to:
1.Demonstrate the standard α-amanitin at concentrations of 25, 50 and 100 μg/ml
2.Determine the deactivation of α-amanitin with 1) 18% acetic acid 2), calcium hydroxide 40 mg/ml, 3) potassium permanganate 20 mg/ml, 4) sodium bicarbonate 20 mg/ml
3.Report the statistical analysis as the mean ± standard deviation (SD) and paired t-test.
The result revealed that potassium permanganate could eliminate 100 percent of the α-amanitin at 25, 50 and 100 μg/ml. Calcium hydroxide, sodium bicarbonate and acetic acid had lower elimination rates at those concentrations: 68.43 ± 2.58 (-71.4, -67.2, .. Read More»
DOI:
10.4172/2157-7145.1000396
Journal of Forensic Research received 1817 citations as per Google Scholar report