Journal of Oncology Translational Research

Sirish Shrestha

Publications

• Research Article
  Apoptotic Effects of Temozolomide and Naturopathic Agents upon Glioblastoma Cells
  Author(s): Albert Magro, Alice Magro, Sirish Shrestha and Kathy BrundageAlbert Magro, Alice Magro, Sirish Shrestha and Kathy Brundage
  
  Temozolomide (TMZ), thymoquinone (TMQ), epigallocatechin gallate (EPIGAL) and staurosporine (STAURO) were used as apoptotic inducing agents acting upon the U87-MG (ATCC, HTB15), LN18 (ATCC, CRL2610) and U118-MG (ATCC, HTB14) glioblastoma multiforme (GBM) cell lines. TMZ is the current drug of choice for primary treatment and adjuvant therapy for recurrent GBM. TMQ and EPIGAL are naturopathic agents, while STAURO is a well-studied apoptotic-inducing agent. The degree and time course of apoptosis were measured by flow cytometry techniques capable of detecting changes in mitochondrial function using the fluorescent dye MitoTracker Deep Red. Phosphatidylserine exposure and plasma membrane permeability were detected simultaneously using violet fluorescent reactive dye (VFRD) in combination with AnnexinV-488. The apoptotic effectiveness of the inducing agents TMZ, TMQ, EPIGAL and STAURO wer.. Read More»
  DOI: 10.4172/2476-2261.1000132

  Abstract PDF