Pharmaceutical Regulatory Affairs: Open Access

Sitty Manohar Babu

Publications

• Research Article
  UPLC Analytical Method Development and Validation for the Simultaneous Estimation of Paracetamol and Caffeine Capsules Dosages Form
  Author(s): Bikash Ranjan Jena, Sitty Manohar Babu, Debi Prasad Pradhan and Suryakanta SwainBikash Ranjan Jena, Sitty Manohar Babu, Debi Prasad Pradhan and Suryakanta Swain
  
  In the present study, a rapid, an accurate and precise Ultra Performance Liquid Chromatography (UPLC) method was developed and validated for simultaneous estimation of paracetamol and caffeine in its capsule dosage form (325 mg and 30 mg) by selecting chromatographic parameters. The UPLC method was developed using 2.1 × 50 mm, reverse phase C18 column (Acquity UPLC ethylene bridge hybrid (BEH) C18 1.7 μm) with mobile phases containing 0.1% w/v H3PO4 and 100% v/v buffer as mobile phase A and methanol: Acetonitrile (50:50) as mobile phase B and water: acetonitrile: H3PO4 (80:20:0.1) as diluent and the run considered as an isocratic elution. Flow rate was 0.5 ml/min with PDA detection at (λmax) 275 n.. Read More»
  DOI: 10.4172/2167-7689.1000186

  Abstract PDF