Egypt
Research Article
Are C-KIT, MMP-9 and Type II Collagen Positive Undifferentiated Cells
Involved in Cartilage Growth? A Description of Unusual Interstitial Type
of Cartilage Growth
Author(s): Soha A Soliman and Hanan H Abd-ElhafeezSoha A Soliman and Hanan H Abd-Elhafeez
Cartilages growth requires activation of perichondrial cells and chondrocyte multiplication. Activated perichondrial cells produce an outer cartilage matrix and in appositional growth and chondrocytes division produce new chondrogenic cells which participate in interstitial growth of cartilage. This study was performed to identify a third type of cartilage growth in skeletal elements of quail embryos at 6, 7, 8, 9, 10, 13, 16, 17 days of incubation. Paraffin sections of the whole embryos were stained using H&E and Safranin O stains. Immunohistochemical staining for C-KIT, matrix metallpoproteinase-9 (MMP-9) and type II collagen was performed. Other samples were processed for scanning and transmission electron microscopy. We found that cells extended form mesenchymal tissue or perichondrium penetrated the cartilage templates of different skeletal elements. The morphological and imm.. Read More»
DOI:
10.4172/2157-7099.1000440
Journal of Cytology & Histology received 2334 citations as per Google Scholar report