Medical Microbiology & Diagnosis

Sophie E. Collier

Publications

• Research Article
  The Role of 16s rDNA PCR in the Diagnosis of Peritoneal Dialysis-Associated Peritonitis
  Author(s): Holly L. Ciesielczuk, Robert J. Shorten, Andrew Davenport, Hala Kandil, Jane E. Carpenter, Timothy D. McHugh and Sophie E. CollierHolly L. Ciesielczuk, Robert J. Shorten, Andrew Davenport, Hala Kandil, Jane E. Carpenter, Timothy D. McHugh and Sophie E. Collier
  
   Introduction: Despite recent advances in Peritoneal Dialysis (PD), peritonitis remains the most common and serious complication. In a significant proportion of patients a pathogen is not cultured. In this study we investigated the use of 16S rDNA PCR to make a bacterial diagnosis. Methods: We used an optimised DNA extraction and 16S rDNA PCR with DNA sequencing to detect pathogens in Peritoneal Dialysis-Associated Peritonitis (PDAP). Results: Seventy-one PD fluids from twenty-four patients were analysed. In suspected cases of PDAP, thirteen out of twenty-one patients had a bacterial pathogen cultured and 16S rDNA PCR with DNA sequencing identified one additional pathogen. However 16S rDNA PCR only detected the pathogen in five of the culture-positive fluids. All follow-up samples were culture-negative, but possible pathogens were identified in three samples by the .. Read More»
  DOI: 10.4172/2161-0703.1000116

  Abstract PDF