Journal of Bioprocessing & Biotechniques

Sreeahila Retnadhas

Publications

• Research Article
  Optimization of Process Conditions for Biotransformation of Caffeine to Theobromine using Induced Whole Cells of Pseudomonas sp
  Author(s): Sreeahila Retnadhas and Sathyanarayana N GummadiSreeahila Retnadhas and Sathyanarayana N Gummadi
  
  The obromine is a metabolic intermediate produced in caffeine degradation pathway by many bacterial species, which has potential applications in food and pharmaceutical industries. Conventional methods of Theobromine production from xanthine involve harsh physical and chemical conditions which are harmful to the environment. To overcome this, we employed biotechnological route to convert caffeine to theobromine by single demethylation reaction using induced cells of Pseudomonas sp. Initially we screened various divalent metal ions for the production of Theobromine by Pseudomonas sp. from caffeine. Co2+ and Ni2+ accumulates 400 and 100 mg/l of theobromine under initial reaction conditions (2 g/l caffeine, 8 g/l cell loading, pH 7.0,30°C). Co2+ was chosen for further optimization of reaction conditions for Theobromine production using response surface methodology. Data were fitted .. Read More»
  DOI: 10.4172/2155-9821.1000178

  Abstract PDF