Medical Microbiology & Diagnosis

Stamatina Levidiotou

Publications

• Research Article
  Utility of Real-Time PCR in the Diagnosis of Primary Epstein-Barr Virus Infection
  Author(s): Constantina Gartzonika, Georgia Vrioni, Efthalia Priavali, Georgios Pappas and Stamatina LevidiotouConstantina Gartzonika, Georgia Vrioni, Efthalia Priavali, Georgios Pappas and Stamatina Levidiotou
  
  Background: Despite the availability of several serological markers, Epstein-Barr virus (EBV) status of some patients is not easily resolved. Objectives: The aim of the present study was to investigate the quantification and the diagnostic utility of EBV DNA detection as an adjunct to serological diagnosis of primary EBV infection. Study Design: Sera from 118 patients referred for suspected primary EBV infection, were tested for heterophile antibodies (HA), IgM antibodies against viral capsid antigen (VCA IgM) and IgG against nuclear antigen (EBNA IgG). A quantitative real time EBV PCR assay (Light Cycler EBV Quant kit) was simultaneously performed in plasma of these patients. Results: EBV DNA was detected in 43 of 46 patients (93.5%) with serologically confirmed primary infection. By performing real time RCR in the remaining 72 samples, 24 additional cas.. Read More»
  DOI: 10.4172/2161-0703.1000118

  Abstract PDF