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Research Article
Optimization of Process Parameters for Cellulase Production by Bacillus licheniformis MTCC 429 using RSM and Molecular Characterization of Cellulase Gene
Author(s): Bhaumik R Dave, Pritesh Parmar, Ankit Sudhir, Ketankumar Panchal and Subramanian RBBhaumik R Dave, Pritesh Parmar, Ankit Sudhir, Ketankumar Panchal and Subramanian RB
World is threaten to energy crisis which has advances research in bioenergy and specifically development of biofuels to replace petroleum products have increased the use of microbial enzyme like cellulases and xylanases as well as amylases for generation of reducing sugars for their conversion into bioethanol. Extensive research has been carried out in this view but alkaline cellulase production and molecular characterization is not studied in detail so far, this study will aid to achieve it. Optimization of fermentation parameters for production of cellulase was evaluated with the help of Response Surface Methodology (RSM) a statistical design, initial pH (9), moisture ratio (1:1) and incubation time (72 h) (run no.4) were found to be ideal parameters for optimum production of cellulase, substrate Jatropha seed cake without any pre-treatment was found to be an ideal source for cellul.. Read More»
DOI:
10.4172/2155-9821.1000212
Journal of Bioprocessing & Biotechniques received 3351 citations as per Google Scholar report