Mataram University,
Jalan Majapahit-62 Mataram, NTB-83125
Indonesia
Research Article
Expression of the Fragment Gene of Predicted Zymogen Granule Protein (G3MZ19) Homologue in the Bali Cattle (Bos Javanicus) Based on MALDITOF/ TOF.MS Analysis of Bali Cattle Saliva
Author(s): Sulaiman Ngongu DepamedeSulaiman Ngongu Depamede
Bali cattle (Bos javanicus) are a type of beef cattle native to Indonesia. Their size is relatively smaller (300-600 kg) than those of Bos taurus, but Bali cattle have high adaptability to tropical conditions, especially against some diseaeses. Efforts have been done to study the physiology of Bali cattle through their saliva as a non-invasive research material. Predicted Zymogen Granule Protein (G3MZ19) has been reported presence in Bali cattle saliva, however until now has not been characterized. In this paper, the results of our studies on the expression and the phylogenetic study of G3MZ19 fragment gene in Bali cattle are presented. PCR was carried out using the primers developed from Bos Taurus G3MZ19 gene (Ludwig NR database). Our studies revealed that G3MZ19 genes were expressed in the genomic DNA of Bali cattle. Results of dendogram with 1000 bootstrap replications indicate th.. Read More»
DOI:
10.4172/2157-7579.1000260
Veterinary Science & Technology received 4472 citations as per Google Scholar report