Journal of Tissue Science and Engineering

Sunao Sadaoka

Publications

• Research Article
  Atelocollagen Enhanced Osteogenesis in a Geometric Structured Beta-TCP Scaffold by VEGF Induction
  Author(s): Kimitoshi Yagami, Sunao Sadaoka, Hiroshi Nakamura, Saho Komatsu, Jun Onodera, Masahiko Suzuki and Yoshinori KubokiKimitoshi Yagami, Sunao Sadaoka, Hiroshi Nakamura, Saho Komatsu, Jun Onodera, Masahiko Suzuki and Yoshinori Kuboki
  
  In order to establish the convertibility of a host for bone augmentation, we herein developed a new honeycombshaped β-tricalcium phosphate (37H) using atelocollagen as a scaffold, which exhibited unique geometric properties for in vitro and in vivo studies. Human mesenchymal stem cells (MSC) were cultured with 37H or atelocollagen-coated honeycomb-shaped β-tricalcium phosphate (Col37H), and their osteoblastic differentiation was then analyzed. Atelocollagen promoted cell adhesion and formation of vessel-like structures in the tunnels of scaffolds of cultured MSC. The mRNA expression levels of type I collagen, osteocalcin, and VEGF were greater in MSC cultured with Col37H than with 37H. Bone generation with Col37H in the rat calvaria was greater than with 37H, and this was attributed to early vessel construction. A large number of blood vessels invaded tunnels from the.. Read More»
  DOI: 10.4172/2157-7552.1000162

  Abstract PDF