Molecular Biomarkers & Diagnosis

Sunil Kadam

Publications

• Research Article
  A Canonical Transforming Growth Factor Beta-Dependent Signaling Pathway is Present in Peripheral Blood Cells of Cancer Patients with Skeletal Metastasis
  Author(s): Sunil Kadam, Ann Louise Cleverly, Mark Farmen, Janet Grondin, Yvelina Ivanova Cox and Michael LahnSunil Kadam, Ann Louise Cleverly, Mark Farmen, Janet Grondin, Yvelina Ivanova Cox and Michael Lahn
  
  Background: Patients with advanced metastatic cancer often have elevated levels of circulating transforming growth factor beta (TGF-β) that is thought to stimulate receptor mediated signaling through phosphorylated SMAD transcription factors in peripheral blood mononuclear cells. Methods: To identify this TGF-β-dependent gene expression profile in cancer patients, we first evaluated a multi-gene expression profile from ex-vivo treated PBMCs with TGF-β1 stimulation. Change in expression, when challenged with a specific TGF-β receptor type kinase inhibitor was then derived to be ligand and inhibitor specific. Once this profile was established, we examined its role in identifying the activation of a canonical TGF-β1 signaling pathway in patient samples. Results: We discovered a 37 gene sub-set whe.. Read More»
  DOI: 10.4172/2155-9929.1000153

  Abstract PDF