Cancer Science & Therapy

Takahumi Masui

Publications

• Research Article
  Non-thermal Effects of Far-Infrared Ray(FIR) on Human Hepatocellular Carcinoma Cells HepG2 and their Tumors
  Author(s): Tatsuo Ishikawa, Jun Ishibashi, Kikuji Yamashita, Shine-Od Dalkhsuren, Kaori Sumida, Takahumi Masui and Seiichiro KitamuraTatsuo Ishikawa, Jun Ishibashi, Kikuji Yamashita, Shine-Od Dalkhsuren, Kaori Sumida, Takahumi Masui and Seiichiro Kitamura
  
  Background: We developed a cell culture CO2 incubatorand a mice rack that can continuously irradiate cells ormurine with FIR. Our goal is to make clear the non-thermaleffect of FIR on HepG2 with these instrumentsmorphologically. Methods: By using them, in vitro , we examined theproliferation of cultured HepG2 cells with hematocytometer,BrdU assay, WST-1 assay, HE staining, Toluidine bluestaining and microarray studies. And in vivo, we measuredthe tumors, observed the sections by IHC, DAPI stainingwith light microscopes and performed microarray studies. Results: Proliferation of HepG2 cells were suppressed(e.g., cell count declined by 34% after 10 days of FIRirradiation), tumor volumes reduced by 86% after 30 daysof FIR irradiation, mRNA of Vascular Endothelial GrowthFactor (V.. Read More»
  DOI: 10.4172/1948-5956.1000012

  Abstract PDF