Journal of Tissue Science and Engineering

Takehisa Matsuda

Publications

• Research Article
  Fabrication Factory for Tubular Vascular Tissue Mimics based on Automated Rolling Manipulation and Thermo-Responsive Polymers
  Author(s): Takehisa Matsuda, Toshihiko Shirota and Daigo KawaharaTakehisa Matsuda, Toshihiko Shirota and Daigo Kawahara
  
  This study presents a novel methodology for fabrication of tubular vascular tissue mimics by an automated factory using thermoresponsive polymers which have temperature-induced reversible phase transition characteristics; Poly(N-Isopropylacrylamide)-Grafted Gelatin (PNIPAM-gelatin), PNIPAM and gelatin. The custom-designed factory consists of a moving tray, a roller unit driven by rack-and-pinion mechanics, and a local temperature-control unit. PNIPAM-gelatin serves as the matrix, providing temperature-dependent adhesion and detachment of cell sheets. We describe two prototypes of vascular tissue mimics: a tubular medial tissue mimic composed of randomly oriented Smooth Muscle Cells (SMCs), and a hierarchically structured vascular tissue mimic (intimal/medical tissue mimics) composed of longitudinally oriented Endothelial Cells (ECs) on the luminal surface and circumfe.. Read More»
  DOI: 10.4172/2157-7552.1000134

  Abstract PDF