Journal of Forensic Research

Tanin Bhoopat

Publications

• Research Article
  Polymorphism of the Microsatellite DXS8377 in a Population of Northern Thai Males
  Author(s): Worraanong Leewattanapasuk, Asaneepond Kunjae, Padchanee Sangthong and Tanin BhoopatWorraanong Leewattanapasuk, Asaneepond Kunjae, Padchanee Sangthong and Tanin Bhoopat
  
  Objective: This research investigated the polymorphism of the X-chromosomal short tandem repeats (X-STR) DXS8377 in Northern Thai males. Methods: Genomic DNA was extracted from blood or buccal cells of 200 unrelated healthy individuals using Nucleospin® Blood or Nucleospin® Tissue (Macherey-Nagel), respectively. The DXS8377 locus was amplified by polymerase chain reaction (PCR) and the PCR product was analyzed by agarose gel electrophoresis. The allelic ladder was constructed and the size of all PCR products was analyzed by polyacrylamide gel electrophoresis using allelic ladder as a marker. The nucleotide sequences of the different-sized PCR products were analyzed to identify the DXS8377 alleles. Lastly, the Polymorphic information content (PIC) and Power of discrimination (PD) of DXS8377 DNA marker were calculated and the analysis of population relationships was performed us.. Read More»
  DOI: 10.4172/2157-7145.1000357

  Abstract PDF