Journal of Bioengineering & Biomedical Science

Tetsuro Wakatsuki

Publications

• Research Article
  Improving Cardiac Action Potential Measurements: 2D and 3D Cell Culture
  Author(s): Neil J Daily, Yue Yin, Pinar Kemanli, Brian Ip and Tetsuro WakatsukiNeil J Daily, Yue Yin, Pinar Kemanli, Brian Ip and Tetsuro Wakatsuki
  
  Progress in the development of assays for measuring cardiac action potential is crucial for the discovery of drugs for treating cardiac disease and assessing cardiotoxicity. Recently, high-throughput methods for assessing action potential using induced pluripotent stem cell (iPSC) derived cardiomyocytes in both two-dimensional monolayer cultures and three-dimensional tissues have been developed. We describe an improved method for assessing cardiac action potential using an ultra-fast cost-effective plate reader with commercially available dyes. Our methods improve dramatically the detection of the fluorescence signal from these dyes and make way for the development of more high-throughput methods for cardiac drug discovery and cardiotoxicity... Read More»
  DOI: 10.4172/2155-9538.1000168

  Abstract PDF