Journal of Computer Science & Systems Biology

Thean Chor Leow

Publications

• Research Article
  The Role of Arg157Ser in Improving the Compactness and Stability of ARM Lipase
  Author(s): Abu Bakar Salleh, Arilla Sri Masayu Abd Rahim, Raja Noor Zaliha Raja Abdul Rahman, Thean Chor Leow and Mahiran BasriAbu Bakar Salleh, Arilla Sri Masayu Abd Rahim, Raja Noor Zaliha Raja Abdul Rahman, Thean Chor Leow and Mahiran Basri
  
  Consensus approach is an efficient strategy to identify hot residue important for compactness and stability of protein. Structure of ARM lipase was modeled to explore the possible effect of critical point mutation towards structure and function. The significant difference of amino acid at position 157 between ARM lipase (Arg157) and other thermostable lipases (Ser157) was targeted as a critical residue. Using YASARA software, Arg157 was substituted to Ser and subsequently the energy minimized. Both ARM and R157S lipases were analyzed by MD simulations at different temperatures (50ºC,60ºC, and 70ºC). MD simulation result showed that R157S lipase had lower value of RMSD, RMSF, solvent accessible surface area (SASA) and radius of gyration than native ARM lipase. It indicated that R157S lipase had higher compactness in the structure leading to e.. Read More»
  DOI: 10.4172/jcsb.1000088

  Abstract PDF