Molecular and Genetic Medicine

Thorsten Bergmann

Publications

• Review Article
  Progress and Problems with Viral Vectors for Delivery of Talens
  Author(s): Thorsten Bergmann, Eric Schulz and Anja EhrhardtThorsten Bergmann, Eric Schulz and Anja Ehrhardt
  
  It has long been envisaged that gene disruption or gene correction in affected target cells can be efficiently conducted in vitro and in vivo and over the recent years several tools for achieving this goal were developed. Designer nucleases such as zinc finger nucleases (ZFNs) were extensively explored and more recently transcription activator-like effector nucleases (TALENs) were introduced for sequence-specific genome engineering in the mammalian genome. ZFNs and TALENs are fusion proteins containing a customized DNA-binding motif for sequence-specific DNA binding linked to a nuclease for introduction of double-stranded DNA breaks. Both systems were explored in mammalian cells using non-viral and viral delivery methods. Herein, we will provide a state-ofthe- art overview of available virus-based delivery systems for sufficient expression of functional TALENs. We will cover the molec.. Read More»
  

  Abstract PDF