Journal of Cytology & Histology

Timothy Chu

Publications

• Research Article
  Use of Ozone for Inactivation of Bacteria and Viruses in Cryostats
  Author(s): Ingo Maier and Timothy ChuIngo Maier and Timothy Chu
  
  The biocidal efficacy of ozone has been investigated in cryostats at -20°C and +30°C and 30-120 min exposure time. Stainless steel coupons were inoculated with 50 μl bacterial or viral test suspension. The tests were performed using Staphylococcus aureus and Mycobacterium terrae as test bacteria and Polyoma virus (Simian virus 40, SV 40). The inoculated coupons were exposed to a combination of ozone and UV generated by UV lamps in the cryostats. To measure the level of disinfection achieved, the number of surviving bacteria recovered from the coupons and, respectively, the virus infectivity titer was determined. Reduction factors were calculated by dividing the initial titer by the titer of recovered viable bacteria or virus. Bactericidal efficacy at -20°C was demonstrated by inactivation of S. aureus by at least 5 l.. Read More»
  DOI: 10.4172/2157-7099.1000428

  Abstract PDF